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Enhancement of inducible‐type NO synthase gene transcription by protein synthesis inhibitors
Author(s) -
Oguchi Shinobu,
Weisz Alessandro,
Esumi Hiroyasu
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80293-9
Subject(s) - cycloheximide , anisomycin , puromycin , protein biosynthesis , messenger rna , gene expression , microbiology and biotechnology , transcription (linguistics) , protein synthesis inhibitor , nitric oxide synthase , gene , reporter gene , biology , promoter , chemistry , biochemistry , enzyme , linguistics , philosophy
Treatment of mouse maerophage‐like RAW 264.7 cells with certain protein synthesis inhibitors is followed by accumulation of the mRNA for the inducible isoiorm of nitric oxide synthase (i‐NOS). The activity of these compounds on the i‐NOS gene in RAW 264.7 cells was analyzed here in detail. Results show that both cycloheximide and anisomycin can efficiently induce i‐NOS mRNA, even when used at concentrations so low ( ) to have only negligible effects on protein synthesis; puromycin, on the other hand, shows only a limited effect on i‐NOS mRNA expression, detectable only when cells are treated with higher concentrations of inhibitor ( ). In RAW 264.7 cells, low concentrations of cycloheximide trigger an immediate‐early gene response, as indicated by induction of c‐ fos and JE mRNAs, and can efficiently activate transcription of transiently transfeeted recombinant reporter genes including either the i‐NOS or the c‐ fos gene promoters.