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Antisense c‐ myc oligonucleotide promotes chondrogenesis and enhances RA responsiveness of mouse limb mesenchymal cells in vitro
Author(s) -
Motoyama Jun,
Eto Kazuhiro
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80292-0
Subject(s) - chondrogenesis , mesenchymal stem cell , retinoic acid , in vitro , oligonucleotide , limb bud , microbiology and biotechnology , chemistry , biology , cellular differentiation , biochemistry , gene , embryo
To examine the role of c‐ myc protein during chondrogenesis, we exposed 11 day p.c. mouse limb mesenchymal cells to the antisense c‐ myc oligonucleotide in micromass culture. The antisense oligonucleotide inhibited the c‐ myc protein expression, and intensely promoted chondrogenesis in the exposed cells. Most of the cells differentiated into cartilaginous cells, whereas they differentiated into cartilaginous and fibrous cells under the control conditions. The antisense oligonucleotide increased the inhibitory efficiency of all‐ trans retinoic acid (RA) to the chondrogenesis. These results suggest that the c‐ myc protein suppress the chondrogenesis and reduces RA responsiveness in the limb mesenchymal cells.