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Cloning of eggplant hypocotyl cDNAs encoding cytochromes P450 belonging to a novel family (CYP77)
Author(s) -
Toguri Toshihiro,
Tokugawa Klazuhisa
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80286-6
Subject(s) - hypocotyl , cloning (programming) , genetics , biology , molecular cloning , complementary dna , computational biology , botany , gene , computer science , programming language
From eggplant hypocotyl tissues we have cloned two closely related cDNAs encoding cytochromes P450 (P450s) by PCR amplification using a primer designed based on the highly conserved sequence among the known eggplant P450s. One cDNA lacks the NH 2 ‐terminal short sequence that is present in the other, full‐length cDNA. The two predicted protein sequences are 71% identical with each other and show less than 30% identity with any other known P450s. It is concluded that these P450s, which are termed CYP77A1 and ‐A2, belong to a hitherto unknown P450 family.