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Site‐specific labelling of the oligosaccharide chains of antibodies
Author(s) -
Schwarz Uwe,
Wunderlich Gerd,
Brossmer Reinhard
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80275-0
Subject(s) - labelling , sialidase , cleave , sialyltransferase , oligosaccharide , chemistry , yield (engineering) , biochemistry , antibody , clostridium perfringens , sialic acid , enzyme , monoclonal antibody , bacteria , biology , neuraminidase , immunology , materials science , genetics , metallurgy
This paper presents a new method for site‐specific labelling of antibodies employing enzymatic reactions without oxidizing or reducing agents. IgG was first treated with immobilized sialidase from Clostridium perfringens to cleave bound NeuAc. CMP‐9‐deoxy‐9‐salizoyl‐NeuAc, an activated sialic acid analogue, was labelled with 131 I via the iodogen‐method in high yields (> 95%). Then the oligosaccharide chains of antibodies were labelled yield with the radioactive NeuAc analogue by transfer using α‐2,6‐sialyltransferase from rat liver in 50%.