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Isoforms of cellular fibronectin and tenascin in amniotic fluid
Author(s) -
Linnala Auli,
von Koskull Harriet,
Virtanen Ismo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80266-1
Subject(s) - fibronectin , blot , gene isoform , immunoprecipitation , amniotic fluid , microbiology and biotechnology , extracellular matrix , chemistry , tenascin c , extracellular , tenascin , biology , biochemistry , pregnancy , fetus , gene , genetics
Amniotic fluid (AF) obtained from second trimester pregnancies presented extradomain (ED) A, B and an oncofetal (onc‐f) domain containing isoforms of cellular fibronectin (cFn) in Western blotting of gelatin‐bound polypeptides and directly of AF. Western blotting after sequential immunoprecipitation suggested at least three Fn molecules: one containing EDA and the onc‐f domain and another minor component distinctly containing all the domains, and a third one only containing EDA. The immunoblotting results for EDA‐cFn and onc‐f‐cFn were closely similar to that for total Fn, whereas in plasma samples of normal and pregnant women only traces of EDA‐cFn and onc‐f‐cFn, but no EDB‐cFn, were found. Western blotting of AF also indicated the presence of three isoforms of tenascin (Tn), M r 190,000 and 280,000 polypeptides earlier found in many cells, and a M r 200,000 polypeptide, novel for AF and not present in plasma. The results suggest a novel extracellular matrix polypeptide composition for AF.