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Expression and stability of recombinant RQ‐mRNAs in cell‐free translation systems
Author(s) -
Ugarov Victor I.,
Morozov Igor Yu.,
Jung Gyoo Y.,
Chetverin Alexander B.,
Spirin Alexander S.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80255-6
Subject(s) - translation (biology) , recombinant dna , chloramphenicol acetyltransferase , messenger rna , dihydrofolate reductase , escherichia coli , microbiology and biotechnology , rna , cell free system , protein biosynthesis , biology , chemistry , gene expression , enzyme , biochemistry , gene , reporter gene
Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell‐free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Qβ. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ‐CAT mRNA.