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Oxidative DNA base damage and antioxidant enzyme activities in human lung cancer
Author(s) -
Jaruga Pawel,
Zastawny Tomasz H.,
Skokowski Jan,
Dizdaroglu Miral,
Olinski Ryszard
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80240-8
Subject(s) - superoxide dismutase , catalase , antioxidant , glutathione peroxidase , dna damage , chemistry , enzyme , dna , lung cancer , biochemistry , glutathione , microbiology and biotechnology , pathology , biology , medicine
We have investigated levels of antioxidant enzymes and free radical‐induced DNA base modifications in human cancerous lung tissues and in their cancer‐free surrounding tissues. Various DNA base lesions in chromatin of lung tissues were measured by gas chromatography‐mass spectrometry. Activities of superoxide dismutase, catalase and glutathione peroxidase were also measured in lung tissues. Higher levels of DNA lesions were observed in cancerous tissues than in cancer‐free surrounding tissues. Antioxidant enzyme levels were lower in cancerous tissues. The results indicate an association between decreased activities of antioxidant enzymes and increased levels of DNA lesions in cancerous tissues. Higher levels of DNA lesions suggest that free radical reactions may be increased in malignant tumor cells.