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Trypsin induces Ca 2+ ‐activated Cl − currents in X. laevis oocytes
Author(s) -
Durieux Marcel E.,
Salafranca Mi.,
Lynch Kevin R.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80198-3
Subject(s) - chemistry , trypsin , biophysics , biochemistry , biology , enzyme
The protease trypsin induces Ca 2+ ‐activated Cl − currents when applied in concentrations as low as 0.1 to defolliculated, voltage clamped X. laevis oocytes. The response is dose‐dependent and specific, as other proteases (chymotrypsin, Lys‐C and Arg‐C), or trypsin pretreated with soybean trypsin inhibitor, did not induce currents. Intracellular trypsin injection did not induce responses. The current does not appear to result from proteolytic activation of the endogenous receptor for lysophosphatidic acid, the only known Ca 2+ ‐mobilizing receptor consistently present in oocytes. These results suggest the presence on the oocyte membrane of a specific receptor for trypsin.