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Induction of phagocytic activity of M1 cells by an inhibitor of vacuolar FT‐ATPase, bafilomycin A 1
Author(s) -
Kinoshita Kuninori,
Hidaka Hiroyoshi,
Ohkuma Shoji
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80195-9
Subject(s) - bafilomycin , cycloheximide , cell culture , chemistry , staurosporine , phagocytosis , microbiology and biotechnology , tyrosine kinase inhibitor , myeloid leukemia , kinase , biology , biochemistry , protein kinase c , apoptosis , cancer research , cancer , autophagy , genetics
Bafilomycin a 1 , a selective inhibitor of vacuolar H + ‐ATPase, time‐and dose‐dependently induced the differentiation of M1 cells, a murine myeloid leukemic cell line, into macrophage‐like cells as revealed by the phagocytosis of polystyrene latex particles. This differentiation was inhibited not only by actinomycin D and cycloheximide but also by ST‐638 (an inhibitor of tyrosine kinase). However, it was affected neither by K‐252a (an inhibitor of C‐kinase) nor by H‐89 (an inhibitor of A‐kinase), in contrast to the M1 cell differentiation induced by leukemia inhibitory factor (LIF). Okadaic acid inhibited both the bafilomycin A 1 ‐induced and LIF‐induced differentiation of M1 cells.