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Comparison of the binding sites of plant ferredoxin for two ferredoxin‐dependent enzymes
Author(s) -
De Pascalis A.R.,
Schürmann P.,
Bosshard H.R.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80194-0
Subject(s) - ferredoxin , ferredoxin thioredoxin reductase , ferredoxin—nadp(+) reductase , enzyme , binding site , biochemistry , reductase , chemistry , chloroplast , stereochemistry , thioredoxin reductase , thioredoxin , gene
Differential chemical modification of acidic residues was used to map the binding site of plant ferredoxin (Fd) for the chloroplast enzyme ferredoxin:thioredoxin reductase (FTR). Binding of FTR to Fd inhibits chemical modification of Fd residues D34, D65, E92, E93, E94 and C‐terminal A97. The binding site demarcated by these residues differs from that for ferredoxin:NADP + reductase (FNR). The FTR site includes C‐terminal residues but not helix 24–31, which is part of the FNR site. Both sites enclose the [2Fe‐2S] cluster.