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Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein
Author(s) -
Takahashi Kojiro,
Hattori Takako,
Nakanishi Tohru,
Nohno Tsutomu,
Fujita Nobuyuki,
Ishihama Akira,
Taniguchi Shigehiko
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80173-8
Subject(s) - gene , promoter , activator (genetics) , escherichia coli , psychological repression , biology , transcription (linguistics) , microbiology and biotechnology , gene product , gene expression , genetics , linguistics , philosophy
In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero‐boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor‐independent upstream activation signals are operating with these promoters.