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Ca 2+ influx evoked by inositol‐3,4,5,6‐tetrakisphosphate in ras ‐transformed NIH/3T3 fibroblasts
Author(s) -
Hashii Minako,
Hirata Masato,
Ozaki Shoichiro,
Nozawa Yoshinori,
Higashida Haruhiro
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80153-3
Subject(s) - inositol , second messenger system , intracellular , extracellular , inositol phosphate , chemistry , inositol trisphosphate , 3t3 cells , bradykinin , stimulation , receptor , biochemistry , biophysics , endocrinology , biology , transfection , gene
Infusion of inositol‐3,4,5,6‐tetrakisphosphate (Ins(3,4,5,6)P 4 ) from the patch pipette into the cytoplasm, produced a biphasic intracellular free Ca 2+ concentration ([Ca 2+ ] i ) increase in ras ‐transformed NIH/3T3 (DT) cells. The Ins(3,4,5,6)P 4 ‐induced increase in DT cells depended upon extracellular Ca 2+ and was enhanced by membrane hyperpolarization. Identical [Ca 2+ ] i increases were observed with intracellular application of inositol‐1,3,4,5‐tetrakisphosphate (Ins(1,3,4,5)P 4 ) and inositol‐1,3,4,6‐tetrakisphosphate but not with inositol‐1,2,4,5‐tetrakisphosphate, inositol‐1,4,5‐trisphosphate or inositol‐1,3,4,5,6‐pentakisphosphate. Stimulation of DT cells with bradykinin increased the levels of Ins(3,4,5,6)P 4 and Ins(1,3,4,5)P 4 . These results suggest that Ins(3,4,5,6)P 4 may serve as a second messenger for continuous Ca 2+ influx along with other tetrakisphosphates downstream from bradykinin receptors in DT cells.