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Photoaffinity cross‐linking of F 1 ATPase from spinach chloroplasts by 3'‐arylazido‐β‐alanyl‐8‐azido ATP
Author(s) -
Schäfer Hans-Jochen,
Rathgeber Gabriele,
Schuhen Achim,
Berzborn Richard J.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80151-7
Subject(s) - spinach , nucleotide , chloroplast , chemistry , atpase , biochemistry , photoaffinity labeling , enzyme , biophysics , biology , binding site , gene
UV irradiation of the ATPase (CF 1 ) from spinach chloroplasts in the presence of 3'‐arylazido‐β‐alanyl‐8‐azido ATP (8,3'‐DiN 3 ATP) results in a nucleotide‐dependent inactivation of the enzyme and in a nucleotide‐dependent formation of α‐β cross‐links. The results demonstrate an interfacial localization of the nucleotide binding sites on CF 1 .