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A phosphorothioate oligonucleotide blocks reverse transcription via an antisense mechanism
Author(s) -
Boiziau Claudine,
Moreau Serge,
Toulmé Jean-Jacques
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80145-2
Subject(s) - rnase h , complementary dna , reverse transcriptase , oligonucleotide , rna , rnase p , microbiology and biotechnology , cleavage (geology) , duplex (building) , biology , chemistry , messenger rna , biochemistry , dna , gene , fracture (geology) , paleontology
We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (⊃100 nM), the cleavage of the RNA template by the retroviral RNase H at the level of the RNA/17PScap duplex accounted for most of the effect, whereas hybrid‐arrested cDNA synthesis by an RNase H‐independent mechanism marginally contributed to the inhibition. Both mechanisms were sequence‐specific. Above 100 nM, the overall cDNA synthesis was reduced in a non‐specific manner.