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Nitric oxide blocks the cell cycle of mouse macrophage‐like cells in the early G 2 +M phase
Author(s) -
Takagi Kuniaki,
Isobe Yuji,
Yasukawa Kiyoshi,
Okouchi Etsuko,
Suketa Yasunobu
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80128-2
Subject(s) - nitric oxide , cell cycle , sodium nitroprusside , cytochalasin b , microfilament , microbiology and biotechnology , flow cytometry , biology , chemistry , biophysics , cell , biochemistry , endocrinology , cytoskeleton
The effects of nitric oxide produced by macrophage‐like cells (Mml) on the cell cycle were investigated. Mml cells lost proliferative activity in the presence of interleukin‐6 (IL‐6) and a subpopulation accumulated in the G 2 + M phase. This level increased in proportion to the incubation time. The DNA content of the cells was slightly lower than that of Mml cells treated with vinbrastine or demecolcine, drugs which block the cell cycle in the M phase. The peak of the early G 2 +M phase was reduced by treatment with N G ‐mono‐methyl‐ l ‐arginine. However, after treatment with exogenous nitric oxide or sodium nitroprusside, the G 0 /G 1 phase increased, but the early‐G 2 +M and the S phase decreased. The flow cytometry pattern in IL‐6‐treated Mml was the same as that of cytochalasin B‐treated Mml. These data suggest that endogenous nitric oxide affects the microfilament system of IL‐6‐treated Mml cells and blocks the cell cycle in the early G 2 +M phase.