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Cloning and functional expression of a cardiac inward rectifier K + channel
Author(s) -
Ishii Kuniaki,
Yamagishi Toshio,
Taira Norio
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80126-6
Subject(s) - complementary dna , inward rectifier potassium ion channel , microbiology and biotechnology , xenopus , cloning (programming) , amino acid , biology , transmembrane domain , northern blot , expression cloning , messenger rna , chemistry , gene , biochemistry , ion channel , receptor , computer science , programming language
We have isolated a cDNA coding for an inward rectifier K + channel (RBHIK1) from rabbit heart. The cloned cDNA encodes a protein of 427 amino acids with two putative transmembrane segments. The primary structure of RBHIK1 is highly homologous to that of IRK1 which is an inward rectifier K + channel recently cloned from mouse macrophage by expression cloning. When expressed in Xenopus oocytes, RBHIK1 current showed strong inward rectification and was inhibited by extracellular Ba 2+ and Cs + . RNA blot analysis revealed the expression of RBHIKl mRNA in various rabbit tissues, especially high level in the ventricular muscle.