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Cloning and characterization of the cDNA encoding rice elongation factor 1β
Author(s) -
Matsumoto Shogo,
Terui Yoshiaki,
Xi Shixiong,
Taira Hideharu,
Ejiri Shin-ichiro
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80125-8
Subject(s) - complementary dna , leucine zipper , open reading frame , elongation factor , amino acid , peptide sequence , biology , cloning (programming) , coding region , eukaryotic translation elongation factor 1 alpha 1 , nucleic acid sequence , microbiology and biotechnology , genetics , biochemistry , gene , rna , ribosome , computer science , programming language
We have cloned and sequenced a cDNA coding for rice elongation factor 1β (EF‐1β). The clone was 1420 bp long and contained an open reading frame coding for 229 amino acids. The overall identity between rice EF‐1β and rice EF‐1β' [Matsumoto, S., Oizumi, N., Taira, H. and Ejiri, S. (1992) FEBS Lett. 311, 46‐48] is 60% at the amino acid sequence level; a higher percent identical residues (81%) were especially observed in the C‐temiinal region. Rice EF‐1β has no conserved phosphorylation site for casein kinase II and no leucine zipper motif, although these motifs are well conserved in EF‐1δ (= β in plants) subunits of animal EF‐1.