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Yeast GAL11 protein stimulates basal transcription in a gene‐specific manner by a mechanism distinct from that by DNA‐bound activators
Author(s) -
Sakurai Hiroshi,
Ohishi Tomoko,
Amakasu Hiroko,
Fukasawa Toshio
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)80098-7
Subject(s) - general transcription factor , transcription (linguistics) , biology , transcription factor , gene , e box , promoter , dna , response element , taf2 , gene expression , microbiology and biotechnology , genetics , linguistics , philosophy
The GAL11 gene encodes an auxiliary transcription factor required for full expression of many, if not all, genes of the yeast Saccharomycescerevisiae . We have recently shown that GAL11/‐encoded protein (GAL111p) enhances basal transcription from the CYC1 promoter in a cell‐free transcription system [(1993) Proc. Natl. Acad. Sci. USA 90, 8382‐8386]. Here we indicate that Ga111p stimulates basal transcription in a gene‐specific manner in vitro. We further suggest that the mechanism underlying the transcriptional stimulation by Ga111p is distinct from that by DNA‐bound activators, since Ga111p stimulated transcription in a reaction system where activators were unable to enhance transcription due to the lack of intermediary factors.