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The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G‐protein‐coupled receptors
Author(s) -
Harteneck Christian,
Obukhov Alexander G.,
Zobel Andrea,
Kalkbrenner Frank,
Schultz Günter
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01455-a
Subject(s) - sf9 , microbiology and biotechnology , g protein coupled receptor , receptor , g protein , second messenger system , biology , chemistry , ion channel , calcium , biochemistry , recombinant dna , gene , organic chemistry , spodoptera
Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl , which are assumed to be involved in calcium influx, serve as model system. trpl expressed in Sf9 cells showed spontanous activity. Hormonal stimulations of calcium influx (detected by fura‐2) and of an outwardly rectifying current were observed in Sf9 cells coinfected with baculoviruses encoding trpl and various heptahelical receptors for histamine, thrombin, and thromboxane A 2 , all known to cause phospholipase C‐β activation in mammalian cells. Although the identity of the G‐proteins and of possible second messengers involved need to be clarified, it is clear that trpl represents a receptor/G‐protein regulated cation channel.