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Identification of two cell cycle regulated genes affecting the β1,3‐glucan content of cell walls in Saccharomyces cerevisiae
Author(s) -
Ram A.F.J.,
Brekelmans S.S.C.,
Oehlen L.J.W.M.,
Klis F.M.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01418-z
Subject(s) - mutant , cell cycle , saccharomyces cerevisiae , gene , biology , microbiology and biotechnology , cell , transcription (linguistics) , genetics , biochemistry , linguistics , philosophy
The Calcofluor white‐hypersensitive mutants cwh52 and cwh53 are severely reduced in β1,3‐glucan. CWH52 was equivalent to GAS1 . CWH53 represented a new gene, located on the right arm of chromosone XII, and predicted to encode a 215 kDa protein with multiple transmembrane domains. The transcription of CWH53 was cell cycle‐dependent and, similar to GAS1/CWH52 , increased in late G 1 , indicating that the formation of β‐glucan is cell cycle‐regulated. Further, in some mutant alleles of both gas1/cwh52 and cwh53 lethal concentrations of Calcofluor induced growth arrest at a specific phase of the cell cycle.