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Prolactin induces growth inhibition and promotes differentiation of CHO cells stably transfected with prolactin receptor complementary DNA
Author(s) -
Big Christophe,
Daniel Nathalie,
Kermabon Anne-Yvonne,
Djiane Jean
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01400-u
Subject(s) - chinese hamster ovary cell , prolactin , transfection , prolactin receptor , cell growth , receptor , clone (java method) , endocrinology , biology , cellular differentiation , hamster , cell culture , medicine , microbiology and biotechnology , prolactin cell , chemistry , dna , gene , biochemistry , hormone , genetics
We have characterized a stable and functional transfectant of the rabbit prolactin receptor in Chinese hamster ovary cells, and investigated the action of prolactin (PRL) on the growth and differentiation of this transfectant (clone E32). PRL induced a significant inhibition of E32 cell proliferation. Growth inhibition correlated with gene induction of the molecular marker of ovarian differentiation cholesterol side chain cleavage P450 (P450 scc ). Both effects were inversely proportional to cell confluence. The limits and potential development of such transfected cellular systems are discussed.