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Molecular characterization and cell cycle‐regulated expression of a cDNA clone from Arabidopsis thaliana homologous to the small subunit of ribonucleotide reductase
Author(s) -
Philipps Gabriel,
Clement Bernadette,
Gigot Claude
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01397-j
Subject(s) - arabidopsis thaliana , complementary dna , biology , ribonucleotide reductase , protein subunit , cdna library , microbiology and biotechnology , gene , homologous chromosome , homology (biology) , clone (java method) , biochemistry , mutant
A cDNA clone isolated from an Arabidopsis thaliana cell suspension library showed highly significant homology to the small subunit of ribonucleotide reductase (R2) from different species. The 340 amino acid‐long deduced putative protein contains all the residues that are important for the enzyme activity and structure. In A. thaliana this enzyme is encoded by a single‐copy gene. In synchronized tobacco BY2 cells the corresponding mRNAs specifically accumulate during the S phase of the cell cycle.