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Oxidized low‐density lipoprotein is cytotoxic to human monocyte‐macrophages: protection with lipophilic antioxidants
Author(s) -
Marchant Christine E.,
Law Nadine S.,
van der Veen Carina,
Hardwick Simon J.,
Carpenter Keri L.H.,
Mitchinson Malcolm J.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01393-f
Subject(s) - cytotoxic t cell , chemistry , monocyte , low density lipoprotein , cytotoxicity , lipoprotein , macrophage , biochemistry , cholesterol , biology , immunology , in vitro
Human monocyte‐macrophages were incubated for 24 h with low‐density lipoprotein (LDL) which had been previously oxidized for varying periods up to 24 h with copper ions, in the presence or absence of dl ‐α‐tocopherol or probucol. The release of radioactivity from cells preloaded with tritiated adenine was used as an assay of toxicity. Toxicity of oxidized LDL increased with duration of copper oxidation and with increasing evidence of lipid oxidation, measured by assay of thiobarbituric acid‐reactive substances and by gas chromatography. Oxidation and toxicity were inhibited by dl ‐α‐tocopherol (200 μM) and probucol (50 μM).