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An A‐form of poly[d(A‐C)] · poly[d(G‐T)] induced by mercury (II) as studied by UV and FTIR spectroscopies
Author(s) -
Garriga Pere,
Garcia-Quintana David,
Manyosa Joan
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01385-e
Subject(s) - polynucleotide , chemistry , fourier transform infrared spectroscopy , stacking , aqueous solution , metal , infrared spectroscopy , absorption spectroscopy , nucleotide , circular dichroism , derivative (finance) , crystallography , stereochemistry , polymer chemistry , organic chemistry , biochemistry , physics , quantum mechanics , financial economics , economics , gene
The conformational changes of poly[d(A‐C)]·poly[d(GT)] induced by Hg(CiO 4 ) 2 in aqueous solution have been studied using UV absorption and fourth derivative spectrophotometries, and FTIR spectroscopy. The UV absorption and fourth derivative spectra reflect changes in the polynucleotide stacking interactions as a result of the metal‐polynucleotide interaction. The fourth derivative spectra do not indicate a Z‐form either at low or at high metal‐to‐polynucleotide ratios. Furthermore, the infrared spectrum at high metal‐to‐polynucleotide ratio has the main features of an A‐form, in contrast with previous CD studies which proposed that the polynucleotide adopts a Z‐form under these conditions. The nature of a different conformation of the polynucleotide induced at low r ‐ratios ( r < 0.2) is discussed.