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The NssBF element, a sequence of the Drosophila melanogaster retrotransposon 1731 potentially implicated in transcriptional repression and replication
Author(s) -
Lacoste Jérôme,
Fourcade-Peronnet Frédérique
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01373-9
Subject(s) - retrotransposon , drosophila melanogaster , psychological repression , replication (statistics) , biology , genetics , transposable element , sequence (biology) , p element , microbiology and biotechnology , gene , drosophilidae , virology , genome , gene expression
The nuclear single‐stranded DNA binding factor (NssBF) has been characterized as a nuclear protein that binds to a 26 nucleotides sequence in the long terminal repeat (LTR) of the Drosophila melanogaster 1731 retrotransposon. This sequence, called NssBF element, was analysed by gel retardation experiments using wild‐type and mutated oligonucleotides. In vitro transcription experiments were performed and suggest that NssBF element binding protein(s) represses transcription through the 1731 promoter. Furthermore, computer assisted sequence comparisons put forward a possible role of this element and/or its associated DNA binding proteins in replication.