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Ha‐Ras stimulates uptake and phosphorylation of ethanolamine: inhibition by wortmannin
Author(s) -
Kiss Zoltan,
Crilly Karan S.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01371-7
Subject(s) - wortmannin , phosphorylation , phosphatidylinositol , ethanolamine , chemistry , phosphatidylethanolamine , kinase , microbiology and biotechnology , biochemistry , phosphatidylcholine , phospholipid , biology , membrane
Transformation of NIH 3T3 fibroblasts by Ha‐Ras resulted in large increases in the phosphorylation of both [ 14 C]ethanolamine (Etn) and [ 14 C]choline (Cho) when these precursors were added to the medium. Wortmannin, an inhibitor of phosphatidylinositol 3‐kinase (P13K), preferentially decreased phosphorylation of externally added Etn in the Ha‐Ras transformed, but not in the untransformed, fibroblasts. However, wortmannin had no effect on the phosphorylation of Etn formed endogenously by phorbol ester‐stimulated hydrolysis of phosphatidylethanolamine. Data suggest that interaction of mutated Ras with PI3K leads to specific stimulation of Etn uptake, followed by nearly quantitative phosphorylation of Etn by a Ras‐activated Cho/Etn kinase.