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Possible involvement of the 440 kDa isoform of ankyrin B in neuritogenesis in human neuroblastoma NB‐1 cells
Author(s) -
Kunimoto Manabu
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01363-6
Subject(s) - ankyrin , neurite , gene isoform , alternative splicing , messenger rna , ankyrin repeat , microbiology and biotechnology , biology , rna splicing , neuroblastoma , gene , cell culture , in vitro , rna , biochemistry , genetics
Two isoforms of brain ankyrin, 440 kDa and 220 kDa ankyrin B , which are products of alternatively spliced pre‐mRNA encoded by a single gene, are both expressed in human neuroblastoma NB‐1 cells. Expression of the polypeptide and mRNA of the larger isoform increased upon induction of neurite outgrowth in NB‐1 cells by dibutyryl cAMP, while those of the smaller isoform were unaffected. The expressed 440 kDa ankyrin B was concentrated at the tip of growing neurites and was partly co‐localized with GAP‐43. These results suggest that 440 kDa ankyrin B is one of the neuronal growth‐associated proteins and provides an interesting example of gene regulation by alternative splicing in neuronal cells.