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Identification of yeast MAS17 encoding the functional counterpart of the mitochondrial receptor complex protein MOM22 of Neurospora crassa
Author(s) -
Masaaki Nakai,
Toshiya Endo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01362-5
Subject(s) - neurospora crassa , mutant , crassa , yeast , biology , gene , saccharomyces cerevisiae , neurospora , microbiology and biotechnology , mitochondrion , genetics , biochemistry
MOM22 of Neurospora crassa was suggested to be required for the transfer of mitochondrial precursor proteins from the receptors to the protein translocation machinery. We isolated a yeast mutant the viability of which depended on the expression of the introduced N. crassa MOM22 gene. The mutant cells showed defects in protein import into mitochondria when the cells were depleted of MOM22. By screening for suppressor genes for the mutant, we could identify the yeast gene MAS17 encoding the functional counterpart of MOM22. The MAS17 gene was found to be essential for the viability of yeast cells.