Premium
Epidermal growth factor induces serine phosphorylation of actin
Author(s) -
van Delft Sanne,
Verkleij Arie J.,
Boonstra Johannes,
van Bergen en Henegouwen Paul M.P.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01359-9
Subject(s) - autophosphorylation , phosphorylation , epidermal growth factor , serine , tyrosine phosphorylation , microbiology and biotechnology , chemistry , tyrosine , actin , intracellular , epidermal growth factor receptor , biochemistry , biology , receptor , protein kinase a
Stimulation of cells by epidermal growth factor induces a rapid polymerisation of actin in the cortical skeleton. Activation of the EGF‐receptor leads to autophosphorylation and to phosphorylation of specific intracellular substrates. Here we show that actin is phosphorylated in vitro and in vivo upon EGF stimulation. Two‐dimensional phospho‐amino acid analysis shows that phosphorylation occurs on serine, not on tyrosine residues.