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The high potential iron‐sulfur protein (HiPIP) from Rhodoferax fermentans is competent in photosynthetic electron transfer
Author(s) -
Hochkoeppler Alejandro,
Ciurli Stefano,
Venturoli Giovanni,
Zani Davide
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01334-w
Subject(s) - chemistry , photosynthesis , electron transfer , photosynthetic reaction centre , electron transport chain , cytochrome , redox , rhodospirillales , photochemistry , purple bacteria , cytochrome c , rhodospirillaceae , sulfur , biophysics , biochemistry , biology , enzyme , inorganic chemistry , mitochondrion , organic chemistry
The functional role of the High Potential Iron‐sulfur Protein (HiPIP) from the photosynthetic bacterium Rhodoferax fermentans was investigated. We demonstrated that the HiPIP increased the rate of light‐induced oxygen reduction mediated by the photosynthetic reaction center (RC); this stimulation reached half‐saturation at [HiPIP]/[RC] ca. 15. The capability of the HiPIP in delivering electrons to the reaction center of Rhodoferax fermentans was demonstrated through kinetic spectrophotometry of cytochrome c ‐556 oxidation in the presence or in the absence of HiPIP. It is concluded that the HiPIP is competent in the photosynthetic electron transfer chain of Rhodoferax fermentans .