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Phosphate regulation of biosynthesis of extracellular RNases of endospore‐forming bacteria
Author(s) -
Znamenskaya L.V.,
Gabdrakhmanova L.A.,
Chernokalskaya E.B.,
Leshchinskaya I.B.,
Hartley R.W.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01316-s
Subject(s) - barnase , rnase p , bacillus amyloliquefaciens , ribonuclease , biochemistry , extracellular , gene , biology , chemistry , microbiology and biotechnology , rna , fermentation
The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp. The regulatory region of the barnase gene, however, is quite different from the other two. In the promoter of the genes for binase and RNase Bp, but not in that for barnase, is a region similar to the Pho box of E. coli . We have established that inorganic phosphate suppresses the synthesis of the binase and RNase Bp, but does not effect the synthesis of barnase.