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A novel peridinin—chlorophyll a protein (PCP) from the marine dinoflagellate Alexandrium cohorticula : A high pigment content and plural spectral forms of peridinin and chlorophyll a
Author(s) -
Ogata Takehiko,
Kodama Masaaki,
Nomura Setsuzo,
Kobayashi Masayuki,
Nozawa Tsunenori,
Katoh Tetzuya,
Mimuro Mamoru
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01314-4
Subject(s) - peridinin , dinoflagellate , pigment , chlorophyll a , chemistry , chlorophyll c , chlorophyll , botany , photosynthetic pigment , photochemistry , fucoxanthin , biology , biochemistry , chlorophyll fluorescence , organic chemistry
A new type of peridinin—chlorophyll a protein (PCP) was isolated from the marine dinoflagellate Alexandrium cohorticula . Unlike previous studies, PCP was obtained as a single component in the presence of a protease inhibitor. The monomer had a molecular mass of 37 kDa with 12 peridinin molecules associated with 2 chl a molecules. This pigment content was much higher than that reported previously. We observed a partial amino acid sequence of the N‐terminus that is novel among photosynthetic pigment—protein complexes. Magnetic circular dichroism clearly indicated that chl a in PCP had monomeric features. Multiple spectral components were suggested for both chl a and peridinin. Based on the high pigment content, the optical properties were compared with those for a reported PCP containing 4 chl a and 1 peridinin.