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A new human leukemia cell 8.2 kDa differentiation factor: Isolation and primary structure
Author(s) -
Kostanyan Irina A.,
Astapova Mariya V.,
Starovoytova Elena V.,
Dranitsina Svetlana M.,
Lipkin Valery M.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01306-3
Subject(s) - complementary dna , microbiology and biotechnology , retinoic acid , cellular differentiation , peptide sequence , cdna library , biology , nucleic acid sequence , northern blot , nucleotide , leukemia , chronic myelogenous leukemia , leukemia inhibitory factor , protein primary structure , cell culture , amino acid , biochemistry , chemistry , gene , genetics , embryonic stem cell
A new 8.2 kDa differentiation factor has been purified to homogeneity from the cultural media of human myelogenous HL‐60 leukemia cells induced by retinoic acid. cDNA clones encoding this factor were isolated from a cDNA library prepared from HL‐60 differentiated cells and their nucleotide sequence has been determined. The deduced amino acid sequence of the differentiation factor molecule consists of 54 amino acid residues. The protein is shown to be glycosylated. It was shown by Northern blot experiments that the level of poly(A) + RNA with a length of 450 nucleotides was higher in differentiated cells than in non‐differentiated cells.