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Suppression of antiproliferative effects of tumor necrosis factor by transfection of cells with human platelet‐derived growth factor B/c‐sis gene
Author(s) -
Aggarwal Bharat B.,
Pocsik Eva,
Totpal Klara,
Ali-Osman Francis
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01299-g
Subject(s) - transfection , tumor necrosis factor alpha , platelet derived growth factor receptor , growth factor , microbiology and biotechnology , biology , cell culture , cancer research , chemistry , endocrinology , receptor , biochemistry , genetics
The growth of cells is determined by the balance between growth‐stimulatory and growth‐inhibitory signals. In the present study, we demonstrate that the transfection of NIH 3T3 cells with a platelet‐derived growth factor ( PDGF‐B/c‐sis ) gene induces resistance to the anticellular effects of tumor necrosis factor (TNF). Human tumor cell lines that express elevated levels of c‐sis (e.g. epidermoid carcinoma, A‐431) are also TNF resistant, whereas those that express no significant levels of this gene (e.g. breast adenocarcinoma, MCF‐7) are TNF sensitive. Transfection of cells with the c‐sis gene leads to down‐modulation of TNF receptors and also a decrease in intracellular glutathione levels. Thus, our results demonstrate that over‐expression of PDGF‐B/c‐sis by certain tumor cells can lead to their protection from the anticellular effects of TNF.