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Nucleotide sequence and analysis of the mouse SPC3 promoter region
Author(s) -
Hanabusa Tadashi,
Ohagi Shinya,
LaMendola Joseph,
Chan Shu Jin,
Steiner Donald F.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01285-7
Subject(s) - biology , promoter , tata box , enhancer , caat box , gene , microbiology and biotechnology , genetics , proinsulin , consensus sequence , start codon , conserved sequence , prohormone convertase , peptide sequence , gene expression , nucleotide , biochemistry , prohormone , insulin , hormone , endocrinology
Insulin is converted from the higher molecular weight proprotein, proinsulin by highly specific proteolytic cleavage at two dibasic amino acid sites. SPC3 and SPC2, two recently identified prohormone convertase that are specifically expressed in β cells and other neuroendocrine cells, appear to be responsible for those cleavages. We have sequenced the 5′‐upstream region of the SPC3 gene and examined its promotor/enhancer activity and most of several deletion mutants in several cell lines. This region contains no CAAT box but has several non‐functional TATA‐like sequences and several putative transcriptional regulatory elements, including AP‐1, Spl and cAMP response elements. These features are not unlike those of the human SPC2 upstream region. In βTC3 insulinoma cells, the sequence between the Eco RI (620 bp) and Nsi I (702 bp) sites seems to be important for gene expression, while the sequence between the Nsi I and Dra I (775 bp) sites may contain strong enhancer element(s).