Premium
Regulation by glucose of the biosynthesis of PC2, PC3 and proinsulin in (ob/ob) mouse Islets of Langerhans
Author(s) -
Martin Sean K.,
Carroll Raymond,
Benig Michelle,
Steiner Donald F.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01284-9
Subject(s) - proinsulin , biosynthesis , islet , prohormone , medicine , endocrinology , stimulation , chemistry , prohormone convertase , pancreatic islets , insulin , biochemistry , biology , enzyme , hormone
The prohormone convertases PC2 and PC3 have been shown to catalyze the processing of proinsulin to insulin in pancreatic β‐cells. In these studies we have compared the effects of glucose on PC2 and PC3 biosynthesis in freshly isolated islets from normal and hyperglycemic (ob/ob) mice. In contrast to normal islets [Alarcón, et al. (1993) J. Biol. Chem. 268, 4276] the biosynthesis of both PC2 and PC3 is stimulated by glucose, parallel to the stimulation of proinsulin in the (ob/ob) islets. Inhibition of PC2 biosynthesis by glucose in normal islet non β‐cells may obscure stimulation of PC2 biosynthesis in normal islet β‐cells.