Premium
Thyrsiferyl 23‐acetate is a novel specific inhibitor of protein phosphatase PP2A
Author(s) -
Matsuzawa Shu-ichi,
Suzuki Teruaki,
Suzuki Minoru,
Matsuda Akio,
Kawamura Takeshi,
Mizuno Yusuke,
Kikuchi Kunimi
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01281-4
Subject(s) - protein phosphatase 2 , phosphatase , protein tyrosine phosphatase , biochemistry , protein subunit , jurkat cells , chemistry , phosphorylation , protein phosphatase 1 , enzyme , serine , threonine , microbiology and biotechnology , biology , t cell , immune system , immunology , gene
Thyrsiferyl 23‐acetate (TF23A), a cytotoxic compound from marine red alga, has been shown to potently and specifically inhibit serine/threonine protein phosphatase 2A (PP2A) with IC 50 values of 4–16 μM, depending on the enzyme concentration. TF23A did not affect activity of protein phosphatase 1 (PP1), 2B (PP2B), 2C (PP2C), or protein tyrosine phosphatases (PTP) up to 1 mM. It inhibited PP2A activity in a crude extract of a human T cell line, Jurkat cell, as well as the purified catalytic subunit. Thus, TF23A proved to be a novel useful probe for clearly distinguishing the activity of PP2A from those of the other protein phosphatases in crude cell extracts and identification of cellular processes that are regulated by PP2A.