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A 66‐kDa protein associated with epidermal growth factor receptor is a proteolytic fragment of phosphoinositide‐specific phospholipase C
Author(s) -
Medvedeva N.D.,
Alexeyev V.Yu.,
Tsupki.V.,
Nikolsky N.N.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01276-8
Subject(s) - proteolytic enzymes , fragment (logic) , chemistry , phospholipase c , receptor , biochemistry , microbiology and biotechnology , biology , enzyme , computer science , programming language
It is shown that in the A431 cells, EGFR is co‐immunoprecipitated with a group of proteins recognized by antibodies to phospholipase Cγ. These are 145‐ and 47‐kDa proteins corresponding to phospholipase Cγ and Nck, respectively, and an unidentified 66‐kDa protein. The association of phosphoinositide‐specific phospholipase Cγ and 66‐kDa protein to EGFR was observed in the A431 cells with or without the EGF treatment. Trypsin peptide maps of these two proteins are similar so it is assumed that the 66‐kDa protein is related to phospholipase Cγ.