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Cloning and sequencing of four structural genes for the Na + ‐translocating NADH‐ubiquinone oxidoreductase of Vibrio alginolyticus
Author(s) -
Beattie P.,
Tan K.,
Bourne R.M.,
Leach D.,
Rich P.R.,
Ward F.B.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01275-x
Subject(s) - vibrio alginolyticus , oxidoreductase , orfs , biology , protein subunit , biochemistry , gene , escherichia coli , nucleic acid sequence , peptide sequence , homology (biology) , microbiology and biotechnology , nadh dehydrogenase , vibrio , enzyme , genetics , open reading frame , bacteria
Oligonucleotide probes based on the N‐terminal amino acid sequences of the NqrA and NqrC subunits were used to clone genes for the Na + ‐dependent NADH‐ubiquinone oxidoreductase complex from Vibrio alginolyticus . Four consecutive ORFs were identified encoding subunit proteins of 48.6, 46.8, 27.7 and 22.6 kDa, respectively (NqrA—D). A further ORF, showing 71% homology to the BolA protein of Escherichia coli , was located upstream. From sequence comparisons, we conclude that the Na + ‐dependent NADH‐ubiquinone oxidoreductase complex of V. alginolyticus is clearly distinct from the corresponding H + ‐dependent enzymes of both prokaryotes and eukaryotes.