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Cdc25 is not the signal receiver for glucose induced cAMP response in S. cerevisiae
Author(s) -
Goldberg Doron,
Segal Marisa,
Levitzki Alexander
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01273-3
Subject(s) - saccharomyces cerevisiae , yeast , cdc25 , gtp' , guanine nucleotide exchange factor , intracellular , microbiology and biotechnology , heterotrimeric g protein , biochemistry , chemistry , receptor , signal transduction , cell , biology , cell cycle , g protein , cyclin dependent kinase 1 , enzyme
The Ras/cAMP pathway in the yeast S. cerevisiae couples the cell cycle of this unicellular organism to the availability of nutrients. Glucose derepressed S. cerevisiae cells respond to glucose addition by an intracellular rise in cAMP. In the prevailing model, yeast Ras plays a similar role to that of heterotrimeric G‐proteins coupled to cell surface receptors. A crucial element of this model is that the exchanger, Cdc25 is activated by glucose. Such activation would result in a glucose‐dependent rise in GTP‐bound Ras concentration. We here show, in contrast to this view, that Cdc25 cannot be the receiver of the glucose signal. We suggest that the Ras‐GTP/cyclase complex is the molecular element directly receiving the signal while Cdc25‐dependent exchange constitutes a prerequisite for complex formation.