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Is Zn 2+ transported by the mitochondrial calcium uniporter?
Author(s) -
Saris Nils-Erik L.,
Niva Kaija
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01256-3
Subject(s) - uniporter , ruthenium red , valinomycin , chemistry , calcium , membrane potential , respiration , biophysics , mitochondrion , inner mitochondrial membrane , membrane , zinc , biochemistry , biology , cytosol , anatomy , enzyme , organic chemistry
Zinc ions were found to inhibit Ca 2+ uptake by rat liver mitochondria driven by succinate respiration but not that by a valinomycin‐induced membrane potential. Zn 2+ at 1 μM or higher concentrations induced a lowering of the membrane potential under the former but not the latter conditions. It is concluded that it is the lowered membrane potential in the presence of Zn 2+ that reduces the rate of respiration‐driven Ca 2+ . Ruthenium red was found to inhibit the uptake of Zn 2+ but had no influence on its action upon the membrane potential. Zn 2+ did not affect the Ruthenium red‐insensitive Ca 2+ efflux. Ca 2+ stimulated the uptake of Zn 2+ . It is concluded that Zn 2+ may be transported by the mitochondrial calcium uniporter but that it may have access to sites required for inhibition of respiration by other routes.