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Molecular cloning, expression and chromosomal localisation of human AMP‐activated protein kinase
Author(s) -
Beri Raj K.,
Marley Anna E.,
See Chee Gee,
Sopwith William F.,
Aguan Kripa,
Carling David,
Scott James,
Carey Frank
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01247-4
Subject(s) - complementary dna , ampk , microbiology and biotechnology , protein kinase a , biology , gene , blot , molecular cloning , immunoprecipitation , genomic dna , kinase , biochemistry
A cDNA encoding rat liver AMP‐activated protein kinase (AMPK) was used to isolate human skeletal muscle AMPK cDNA clones. Human AMPK cDNA is more than 90% homologous to the rat sequence and predicts a protein of molecular mass 62.3 kDa, which closely agrees with the mass observed in Western blots of human tissues. AMPK antibodies were also shown to immunoprecipitate AMPK from human liver extracts. A cDNA probe was used to identify a 9.5kb transcript in several human tissues and to isolate human genomic clones. PCR mapping of rodent/human hybrid cell lines localised the human AMPK gene to chromosome 1, and fluorescent in situ hybridisation with a human genomic clone was used to sub‐localise the human AMPK gene to 1p31.