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Changes in the NAD(P)H concentration caused by addition of nitrogenase ‘switch‐off’ effectors in Rhodospirillum rubrum G‐9, as measured by fluorescence
Author(s) -
Norén Agneta,
Nordlund Stefan
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01233-4
Subject(s) - rhodospirillum rubrum , nitrogenase , nad+ kinase , chemistry , fluorescence , redox , photochemistry , biochemistry , nitrogen fixation , inorganic chemistry , nitrogen , enzyme , organic chemistry , physics , quantum mechanics
The effect of nitrogenase ‘switch‐off’ effectors on the concentration of NAD(P)H in Rhodospirillum rubrum G‐9 was investigated by fluorescence. A rapid decrease in fluorescence was observed when cells, either N 2 ‐grown or nitrogen‐starved, were subjected to the effectors, but not when sodium chloride or Tris buffer was added. No effects on the fluorescence were observed in non‐nitrogen fixing cultures except when NAD + was added. The results strongly indicate that the redox state of the pyridine nucleotide pool affects the control of the regulation of nitrogenase activity in R. rubrum .