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Monoclonal antibodies against metalloporphyrins. Specificity of interaction with structurally different metalloporphyrins
Author(s) -
Savitsky Alexander P.,
Demcheva Marina V.,
Mantrova Ekaterina Yu.,
Ponomarev Gely V.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01221-0
Subject(s) - monoclonal antibody , paratope , chemistry , antibody , conjugated system , antigen , microbiology and biotechnology , monoclonal , biochemistry , biology , immunology , organic chemistry , polymer
Monoclonal antibodies against Pd‐coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd‐coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd‐coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd‐coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrin.