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Production and characterization of monoclonal anti‐idiotypic antibodies to muramylpeptide
Author(s) -
Mareeva T.Yu.,
Kotova O.V.,
Golovina T.N.,
Nesmeyanov V.A.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01220-2
Subject(s) - monoclonal antibody , antibody , microbiology and biotechnology , chemistry , stain , immunization , lysis , staining , biology , immunology , genetics
Hybridomas producing monoclonal anti‐idiotypic antibodies (anti‐id MAbs) to N ‐acetylglucosaminyl‐β1‐4‐ N ‐acetylmuramyl‐alanyl‐ d ‐isoglutamine (GMDP) were developed. Three clones of hybridomas demonstrated the properties characteristic for the Ab2β type of anti‐id antibody: they bound to Fab‐fragments of high‐affinity MAb to GMDP; dose‐dependent inhibition of this binding by GMDP was observed; immunization of mice with these MAbs resulted in production of GMDP‐specific antibodies. When these antibodies were used to stain blots from SDS‐PAGE of macrophage lysate, the same receptor proteins were specifically stained as upon staining with 125 I‐labelled GMDP derivative.