Premium
Biochemical characterization of glucosaminylmuramyldipeptide binding sites of murine macrophages
Author(s) -
Golovina T.N.,
Sumaroka M.V.,
Samokhvalova L.V.,
Shebzukhov Yu.V,
Andronova T.M.,
Nesmeyanov V.A.
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01219-9
Subject(s) - radioligand , photoaffinity labeling , chemistry , cell , affinity label , microbiology and biotechnology , biochemistry , binding site , biology
By using radioligand analysis, murine peritoneal macrophages were shown to express several hundred high‐affinity cell surface GMDP‐binding sites ( K a 350 pM). Photoaffinity labeling followed by SDS‐PAGE enabled us to identify 32–34 and 38 kDa proteins inside these cells that bound GMDP specifically.