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Expression of cytochrome P ‐450 3A in HT29‐MTX cells and Caco‐2 clone TC7
Author(s) -
Carrière Véronique,
Lesuffleur Thécla,
Barbat Alain,
Rousset Monique,
Dussaulx Elisabeth,
Costet Philippe,
de Waziers Isabelle,
Beaune Philippe,
Zweibaum Alain
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01199-0
Subject(s) - cytochrome , clone (java method) , microbiology and biotechnology , caco 2 , biology , microsome , messenger rna , cytochrome p450 , cell culture , enzyme , gene , biochemistry , genetics
HT‐29 sublines and Caco‐2 clones were analyzed for the expression of cytochrome P ‐450 3A. The enzyme was found to be expressed in differentiated HT‐29 cells selected by resistance to methotrexate and in one of seven Caco‐2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P ‐450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.