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Inositol phospholipid metabolism in Xenopus oocytes mediated by endogenous G o and G i proteins
Author(s) -
Kasahara Jiro,
Sugiyama Hiroyuki
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01170-2
Subject(s) - xenopus , metabotropic glutamate receptor , receptor , biology , phospholipase c , metabotropic glutamate receptor 1 , microbiology and biotechnology , g protein , endogeny , metabotropic receptor , inositol , biochemistry , chemistry , glutamate receptor , gene
To characterize G‐proteins which mediate the signal transduction from ligand stimulated receptor to phospholipase C (PLC), we injected antisense DNAs complementary to Xenopus G o α or G i‐1 α to suppress these endogenous G‐proteins, together with the mRNAs encoding metabotropic glutamate receptor 1 (mGluR1), 5 (mGluR5) or with M1 type muscarinic receptor into oocytes. Receptor‐stimulated chloride current responses were reduced by the suppression of Xenopus G o α regardless of the types of receptors. However, injection of G i‐1 antisense DNA resulted in the reduction of M1‐stimulated responses but not mGluR‐stimulated responses. These results suggested that all these receptors could use G o α and M1 receptors, but not mGluRs, could also use G i‐1 proteins, to activate PLC in Xenopus oocytes.