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Glycotripeptides are released by yeast but not by mammalian microsomes
Author(s) -
van Leyen Klaus,
Wiedmann Martin,
Wieland Felix
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01167-2
Subject(s) - endoplasmic reticulum , microsome , yeast , golgi apparatus , spheroplast , microbiology and biotechnology , biochemistry , membrane , chemistry , biology , in vitro , escherichia coli , gene
Glycotripeptides generated in vivo in the endoplasmic reticulum (ER) have been used as markers to assess the rate of vesicular bulk flow from the ER via the Golgi apparatus to the plasma membrane in mammalian cells. The applicability of such glycotripeptides as markers for bulk flow along this pathway has been questioned by a report on non‐vesicular release of glycotripeptides from yeast semi‐intact spheroplasts. We have therefore investigated direct release of glycotripeptides from yeast and from mammalian microsomes and report here that such release is specific to the yeast system and cannot be detected in mammalian microsomes.