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Isolation of a cDNA clone encoding a novel membrane protein expressed in lymphocytes
Author(s) -
Shimizu Yoshifumi,
Ogawa Hiroyasu,
Oka Yoshihiro,
Mizuno Ryuzo,
Sakoda Saburo,
Kishimoto Tadamitsu,
Sugiyama Haruo
Publication year - 1994
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(94)01164-8
Subject(s) - complementary dna , suppression subtractive hybridization , microbiology and biotechnology , biology , clone (java method) , signal peptide , cdna library , peptide sequence , gene , biochemistry
By subtractive hybridization using single‐stranded phagemids with directional inserts, we isolated a mouse cDNA clone, LSM‐1, from temperature‐sensitive Abelson virus‐tranformed immature B cells whose differentiation was being induced after the shift from the permissive (35°C) to the non‐permissive temperature (39°C). LSM‐1, which encodes an as yet unknown peptide of 197 amino acids, has a putative signal sequence and a trans‐membrane region, and is expressed in B‐ and T‐cell lines, in spleen, thymus, and bone marrow of adult mice, and in embryos.